181 to 190 of 196 Results
Jan 9, 2025
Love, Celina; Steinkühler, Jan; Gonzales, David Thomas; Yandrapalli, Naresh; Robinson, Tom; Dimova, Rumina; Tang, T-Y Dora, 2025, "Reversible pH-Responsive Coacervate Formation in Lipid Vesicles Activates Dormant Enzymatic Reactions data", https://doi.org/10.17617/3.DC72LD, Edmond, V1
In situ, reversible coacervate formation within lipid vesicles represents a key step in the development of responsive synthetic cellular models. Herein, we exploit the pH responsiveness of a polycation above and below its pKa , to drive liquid-liquid phase separation, to form single coacervate droplets within lipid vesicles. The process is complete... |
Weigert, Martin; Schmidt, Uwe; Boothe, Tobias; Müller, Andreas; Dibrov, Alexandr; Jain, Akanksha; Wilhelm, Benjamin; Schmidt, Deborah; Broaddus, Coleman; Culley, Siân; Rocha-Martins, Mauricio; Segovia-Miranda, Fabián; Norden, Caren; Henriques, Ricardo; Zerial, Marino; Solimena, Michele; Rink, Jochen; Tomancak, Pavel; Royer, Loïc Alain; Jug, Florian; Myers, Eugene, 2025, "Content Aware Image Restoration: Pushing the Limits of Fluorescence Microscopy data", https://doi.org/10.17617/3.FDFZOF, Edmond, V1
Fluorescence microscopy is a key driver of discoveries in the life-sciences, with observable phenomena being limited by the optics of the microscope, the chemistry of the fluorophores, and the maximum photon exposure tolerated by the sample. These limits necessitate trade- offs between imaging speed, spatial resolution, light exposure, and imaging... |
Jan 9, 2025
Drobot, Björn; Iglesias-Artola, Juan M.; Le Vay, Kristian Kyle; Mayr, Viktoria; Kar, Mrityunjoy; Kreysing, Moritz; Mutschler, Hannes; Tang, T-Y Dora, 2025, "Compartmentalised RNA catalysis in membrane-free coacervate protocells data", https://doi.org/10.17617/3.ECC0GF, Edmond, V1
Phase separation of mixtures of oppositely charged polymers provides a simple and direct route to compartmentalisation via complex coacervation, which may have been important for driving primitive reactions as part of the RNA world hypothesis. However, to date, RNA catalysis has not been reconciled with coacervation. Here we demonstrate that RNA ca... |
Jan 9, 2025
Weigert, Martin; Myers, Eugene; Kreysing, Moritz, 2025, "Biobeam - Rigorous wave-optical simulations of light-sheet microscopy data", https://doi.org/10.17617/3.CGO4I3, Edmond, V1
Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables to simulate operational light-sheet microscopes by combining data from 105–106 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of thes... |
Jan 9, 2025
Brando, Manuel, 2025, "Khanenko_et_al_PRB_2025", https://doi.org/10.17617/3.59LQBY, Edmond, V1
Datasets for publication P. Khanenko et al., Phys. Rev. B (2025). |
Jan 9, 2025
Chakraborty, Debojyoti; Buchholz, Frank; Kappei, Dennis; Theis, Mirko; Nitzsche, Anja; Ding, Li; Paszkowski-Rogacz, Maciej; Surendranath, Vineeth; Berger, Nicolas; Schulz, Herbert; Saar, Kathrin; Hubner, Norbert, 2025, "Combined RNAi and localization for functionally dissecting long non-coding RNAs data", https://doi.org/10.17617/3.BDDOHF, Edmond, V1
Whereas methods to comprehensively study cellular roles of protein-coding genes are available, techniques to systematically investigate long noncoding RNAs (lncRNAs), which have been implicated in diverse biological pathways, are limited. Here we report combined knockdown and localization analysis of noncoding RNAs (c-KLAN) that merges functional c... |
Jan 9, 2025
https://orcid.org/0000-0002-7099-1204; Greenan, Garrett; Prohaska, Sonja J; Baum, Daniel; Hege, Hans-Christian; Müller-Reichert, Thomas; Hyman, Anthony; Verbavatz, Jean-Marc, 2025, "Automated tracing of microtubules in electron tomograms of plastic embedded samples of Caenorhabditis elegans embryos.", https://doi.org/10.17617/3.QNT1RY, Edmond, V1
The ability to rapidly assess microtubule number in 3D image stacks from electron tomograms is essential for collecting statistically meaningful data sets. Here we implement microtubule tracing using 3D template matching. We evaluate our results by comparing the automatically traced centerlines to manual tracings in a large number of electron tomog... |
Jan 9, 2025
Jaensch, Steffen; Decker, Markus; Hyman, Anthony; Swenson, Eugene, 2025, "Automated tracking and analysis of centrosomes in early Caenorhabditis elegans embryos", https://doi.org/10.17617/3.HEYMFZ, Edmond, V1
Motivation: The centrosome is a dynamic structure in animal cells that serves as a microtubule organizing center during mitosis and also regulates cell-cycle progression and sets polarity cues. Automated and reliable tracking of centrosomes is essential for genetic screens that study the process of centrosome assembly and maturation in the nematode... |
Christopher Gell; Berndt, Michael; Enderlein, Jörg; Diez, Stefan, 2025, "Supporting Information for TIRF microscopy evanescent field calibration using tilted fluorescent microtubules.", https://doi.org/10.17617/3.GMINAX, Edmond, V1
Total internal reflection fluorescence microscopy has become a powerful tool to study the dynamics of sub-cellular structures and single molecules near substrate surfaces. However, the penetration depth of the evanescent field, that is, the distance at which the excitation intensity has exponentially decayed to 1/e, is often left undetermined. This... |
Jan 9, 2025
Crowley, John, 2025, "Cafe-Africa data as used in Crowley et al 2025 Peroxy acetyl nitric anhydride (PAN) and peroxy acetic acid (PAA) over the Atlantic west of Africa during CAFE-Africa and the influence of biomass-burning", https://doi.org/10.17617/3.2F0J4Z, Edmond, V1
Time series of mixing ratios of PAN, PAA, NO, NO2, CO, CH3CN and black-carbon as well as aircraft location for each flight analysed during the airborne HALO campaign "CAFE-Africa" in 2018 as used in Crowley et al (Peroxy acetyl nitric anhydride (PAN) and peroxy acetic acid (PAA) over the Atlantic west of Africa during CAFE-Africa and the influence... |
